PUBLICATIONS
Selected publications by the staff of the Department of Reproductive Biotechnology and Cryoconservation of the National Research Institute of Animal Production, dealing with semen, oocyte and embryo cryopreservation as well as advanced, comprehensive methods for semen evaluation.
Trzcińska M., Samiec M. (2021). Ex situ conservation and genetic rescue of endangered Polish cattle and pig breeds with the aid of modern reproductive biotechnology. Annals of Animal Science, DOI: 10.2478/aoas-2021-0046.
Trzcińska M., Kralka-Tabak M. (2021). Cryopreservation and storage of honeybee drone semen–technological aspects and legal regulations. Roczniki Naukowe Zootechniki, 48: 29-37.
Trzcińska M., Bryła M. (2020). A new sperm selection criterion for cryopreservation of boar semen. Annals of Animal Science, DOI: 10.2478/aoas-2020-0095.
Trzcińska M., Bryła M. (2020). Directions and possibilities of modification of cryopreservation methods and assessment of boar semen quality. Scientific editor: Monika Trzcińska, Balice, The National Research Institute of Animal Production, pp.1-76.
Trzcińska M., Bryła M. (2020). The role of the National Research Institute of Animal Production in the development of cryopreservation technology of livestock biological material and its use in ex situ conservation. Roczniki Naukowe Zootechniki, 47: 29-36.
Trzcińska M., Bryła M. (2019). Cryopreservation of biological material of farm animals (https://lifescience.pl/miesiac-partnera-klastra/kriokonserwacja-materialu-biologicznego-zwierzat-gospodarskich/).
Gogol P., Bryła M., Trzcińska M., Bochenek M. (2019). Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders. Pol. J. Vet. Sci., 22: 177-179.
Trzcińska M., Bryła M., Gajda B., Gogol P. (2015). Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene. Theriogenology, 83: 307–313.
Trzcińska M., Bryła M. (2015). Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants. Pol. J. Vet. Sci., 18: 473–480.
PATENTS AND IMPLEMENTATION INSTRUCTIONS
Patents obtained
1. Trzcińska M., Bryła M. (2018). Patent No PL 228192 for the invention “Diluent for boar semen freezing and the method of freezing semen”, granted by the Polish Patent Office.*
*Implementation agreement ZZP/184/2018 concluded on 26 July 2018 between the National Research Institute of Animal Production and the Experimental Station of the National Research Institute of Animal Production Żerniki Wielkie Ltd., carried out in the years 2018-2019, concerning implementation of the patented invention.
2. Gajda B., Smorąg Z., Wieczorek J. (2008). Patent No PL 199290 for the invention “Method of transplantation of vitrified pig blastocysts”, granted by the Polish Patent Office.
Patent applications
Gajda B., Smorąg Z., Wieczorek J., Poniedziałek-Kempny K., Rajska I. (2020). Application No P.432157 for the invention “Culture method for immature pig oocytes resulting in their efficient cryopreservation”.
Bryła M., Trzcińska M. (2019). Application No P. 432175 for the invention “Method for preparing an insemination dose of cryopreserved boar semen”.
Gogol P., Cegła M. (2018). Application No P.425455 for the invention “Diluent for ram semen freezing and the method of freezing semen”.
Implementation instructions
Gajda B., Smorąg Z. (2018). “Cryopreservation of pig oocytes”, National Research Institute of Animal Production. Press: pp.1-20. ISBN 978-83-7607-324-8.
Trzcińska M., Bryła M., Gajda B. (2014). “Intrauterine insemination of gilts with cryopreserved boar semen”, National Research Institute of Animal Production. Press: pp.1-12. ISBN 978-83-7607-249-4.
Trzcińska M., Bryła M., Gogol P., Cegła M. (2013). “Cryopreservation of boar semen”, National Research Institute of Animal Production. Press: pp.1-12. ISBN 978-83-7607-296-3.
CALENDAR OF MAJOR ACHIEVEMENTS IN THE CRYOPRESERVATION OF THE NATIONAL RESEARCH INSTITUTE OF ANIMAL PRODUCTION
1977 – transfer of frozen sheep embryos and generation of lambs, the first in Poland and one of the few in the world
1978 – transfer of frozen cattle embryos and generation of the first calves in Poland
1989 – transfer of vitrified rabbit embryos; the first rabbits obtained using this method in the world
2003 – first successful transfer of vitrified pig embryos in Poland, resulting in piglets born
2015 – surgical insemination of gilts with cryopreserved semen and generation of live piglets
2017 – standard insemination of gilts with cryopreserved semen and generation of live piglets in litter
AWARDS AND DISTINCTIONS
Award from the Minister of Agriculture and Rural Development
In 2016, Prof. Dr hab. Barbara Gajda and Prof. Dr hab. Zdzisław Smorąg were honoured with the Award from the Minister of Agriculture and Rural Development for outstanding national achievements of importance to the implementation of progress in agriculture. The achievement consisted in production of piglets following transfer of vitrified oocytes. This attainment is an important contribution to international achievements in this area.
Distinction from the Polish Academy of Sciences
In 2011, Dr hab. Marek Romek (Jagiellonian University), Prof. Dr hab. Barbara Gajda, Dr Ewa Krzysztofowicz-Romek (Jagiellonian University) and Prof. Dr hab. Zdzisław Smorąg received a Distinction from Division II of Biological and Agricultural Sciences of the Polish Academy of Sciences for a series of research studies “Lipids in pig oocytes and embryos – new methods for identification and content regulation, and the implications for biotechnology”.
First Award from the Minister of Science and Higher Education
In 2008, Prof. Dr hab. Zdzisław Smorąg was honoured with the prize for outstanding achievements in the economic development.